We develop complex nanostructured topologies for cellular interfaces and investigations. Using a bottom-up approach, we design and synthesize nanomaterial-based platforms that allow tailoring of the materials’ optical, electrical and chemical properties. This approach allows a unique formation of structures in one-, two- and three-dimensional geometries that will be used in further experimental designs.
The extraordinary electrical properties, high flexibility and high transparency of graphene have made it an excellent candidate for developing electrodes to stimulate and record from electrogenic cells. However, to enable long term stable interface of graphene based electrodes with cells, it is essential to investigate the toxicity of graphene. Therefore, we investigated the effect of graphene on both non-neuronal and neuronal cells under real-life physiological conditions using two of the most reliable and sensitive intracellular markers of toxicity and stress. Our results indicate that graphene not only promotes cell adhesion and cell proliferation of both non-neuronal and neuronal cells, but also has no detectable adverse effect on cell stress, making it an excellent candidate for cellular interfaces.
Understanding the effect of 2-D Graphene on Cell adhesion, proliferation and stress
The rationale behind incorporating nanostructures and forming a nano composite material is to compensate for materials intrinsic limitations such as mechanical properties, electrical conductivity, the absence of adhesive and microenvironment-defining moieties, and the inability of cells to self-assemble to three-dimensional tissues.
The conceptualized nanostructured platform for cellular interfaces
Human mesenchymal stem cells (hMSC) cultured on nanocomposite nanofibers